A highly selective fluorescent probe for hypochlorite and its endogenous imaging in living cells.
نویسندگان
چکیده
An oxime based fluorescent probe has been designed and synthesized, which detects free as well as enzymatically generated hypochlorite with a low detection limit and high sensitivity. In addition, the probe was successfully utilized for the monitoring of endogenously produced hypochlorite in LPS stimulated cell lines, C6 glioma and BV2 microglia.
منابع مشابه
A fast-responsive mitochondria-targeted fluorescent probe detecting endogenous hypochlorite in living RAW 264.7 cells and nude mouse.
A very fast-responsive fluorescent probe PZ-Py for imaging mitochondrial HClO/ClO(-), with a relatively long emission wavelength, was prepared. The limit of detection was evaluated to be 17.9 nM. Moreover, the probe PZ-Py was successfully applied in the imaging of endogenous HClO/ClO(-) in the mitochondria of RAW 264.7 cells and living nude mouse.
متن کاملReal-time detection of hypochlorite in tap water and biological samples by a colorimetric, ratiometric and near-infrared fluorescent turn-on probe.
In this paper, we report a highly selective and sensitive ratiometric NIR fluorescent probe that can be used for real-time detection of the biologically important hypochlorite with colorimetric and significant NIR fluorescent turn-on signal changes at NIR excitation wavelength. In addition, experiments showed that this probe can be applied to detect hypochlorite in tap water, serum samples, and...
متن کاملA highly selective and sensitive near-infrared fluorescent probe for imaging of hydrogen sulphide in living cells and mice
Hydrogen sulphide (H2S), the third endogenous gaseous signalling molecule, has attracted attention in biochemical research. The selective detection of H2S in living systems is essential for studying its functions. Fluorescence detection methods have become useful tools to explore the physiological roles of H2S because of their real-time and non-destructive characteristics. Herein we report a ne...
متن کاملA fast-response, highly sensitive and selective fluorescent probe for the ratiometric imaging of nitroxyl in living cells.
A fast-response, highly sensitive and selective fluorescent probe with the 2-(diphenylphosphino)benzoate moiety as a recognition receptor for the ratiometric imaging of nitroxyl in living cells was first developed.
متن کاملDevelopment of an ICT-based ratiometric fluorescent hypochlorite probe suitable for living cell imaging.
We have judiciously constructed a novel ICT-based ratiometric OCl(-) probe capable of ratiometric imaging in the live cells based on the new OCl(-)-promoted de-diaminomaleonitrile reaction.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemical communications
دوره 50 80 شماره
صفحات -
تاریخ انتشار 2014